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Low Oxygen Concentration Enhances Chondrogenesis and Secretome Production in Mesenchymal Stem Cells Cultured in a Three-Dimentional Decellularized Bovine Cartilage Scaffolds

Abstract Introduction: Stem cells hold promise for tissue repair due to their secretome, which is influenced by the microenvironment. To improve cartilage regeneration, a three-dimensional (3D) natural cartilage scaffold system has been developed to create a more chondrogenic secretome. As human cartilage is avascular, a hypoxic environment may better mimic in vivo conditions. This study investigates if oxygen levels (normoxic vs. hypoxic) affect MSC chondrogenic potential and secretome composition in this 3D scaffold. Methods: This study used a randomized time series design to investigate how oxygen levels affect the transformation of MSCs into cartilage cells within a 3D cartilage scaffold. A control group (C) was grown in a standard medium. Two experimental groups, P1 and P2, were cultured n a 3D cartilage scaffold under normal (21%) and hypoxic (5%) oxygen concentration. respectively.

Natural Cartilage-Derived Scaffolds for 3D Mesenchymal Stem Cell Culture: Promoting Chondrogenesis and Modulating Secretome Composition

Abstract: Introduction: The regenerative potential of stem cells lies in their secretome, a collection of signaling molecules whose composition is shaped by the cellular microenvironment and external stimuli. To enhance cartilage regeneration, a 3D culture system using a natural cartilage scaffold has been developed to create a more chondrogenic secretome. This study investigates the chondrogenic differentiation of mesenchymal stem cells (MSCs) cultured in a decellularized bovine cartilage scaffold and analyzes the resulting secretome's composition. Methods: This study employed a randomized time series design to examine MSCs chondrogenic differentiation. A control group was cultured in standard 2D conditions, while two experimental groups were cultured in either 2D medium supplemented with chondrocyte differentiation medium (positive control) or a 3D decellularized bovine cartilage scaffold.

Heimioporus sp. (Pelawan mushroom) as source of nutrition and antioxidant

Abstract Edible mushrooms are nutrient-dense, being low in calories yet rich in fiber, protein, vitamins, minerals, and bioactive compounds. The Pelawan mushroom (Heimioporus sp.), an edible variety native to Indonesia, specifically Bangka Belitung, has remained largely unexplored as a bioactive resource. This study aimed to investigate the proximate composition, mineral and vitamin content, and antioxidant potential of Pelawan mushrooms. Samples were collected from Belitung Island, Indonesia, during the rainy season in April 2024. Proximate analysis revealed that Pelawan mushrooms are low in fat but high in protein, fiber, and vitamin A, along with an abundance of essential minerals and vitamins. Extraction using solvents such as n-hexane, ethyl acetate, methanol, and water yielded 3.15 %, 1.26 %, 15.82 %, and 19.11 %, respectively.

The varied Q creatinine in multi ethnics population and impact of adopting three different estimated glomerular filtration rates based on creatinine in adult populations: a call for performance validation

Background: The determination of kidney function is commonly done by estimating the glomerular filtration rate (eGFR) using serum creatinine levels. Various eGFR formulas, including the recently developed European Kidney Function Consortium (EKFC) and the Chronic Kidney Disease Epidemiology Collaboration(CKD-EPI), have been adopted and are commonly utilized in clinical settings. Nevertheless, the extent of acceptance among these formulations in the multi ethnics populace is still undetermined. Thus, this study aimed to evaluate the performance of these formulations across different glomerular filtration rate categories in the adult population. Methods: The research involved a total of 9,557 individuals (median age of 40 years and 85% being male) who underwent routine medical examinations. Enzymatic or modified Jaffe techniques were employed to measure serum creatinine levels.

Blood Q Creatinine Based on Sex and Age in Healthy Indonesian Geriatrics

ABSTRACT Median blood creatinine (Qcr) is necessary for an accurate evaluation of geriatric renal function. Nonetheless, there is a significant void in existing literature concerning creatinine reference data unique to the elderly. To improve diagnostic precision and clinical decision-making in this susceptible group, this study developed a corresponding Q model for serum creatinine in geriatric patients stratified by age and sex. To determine the Qcr serum and reference range creatinine concentrations, 913 healthy elderly patients (452 males and 461 females) undergoing routine examinations at clinical laboratories were studied. Creatinine concentration reference intervals were divided into 3 age groups, namely: 60-69 years, 70-79 years, and >80 years. The median and percentiles p2.5 (lower reference limit/LRL) and p97.5 (upper reference limit/URL) were determined by the study.