#cartilage

Natural Cartilage-Derived Scaffolds for 3D Mesenchymal Stem Cell Culture: Promoting Chondrogenesis and Modulating Secretome Composition

Abstract: Introduction: The regenerative potential of stem cells lies in their secretome, a collection of signaling molecules whose composition is shaped by the cellular microenvironment and external stimuli. To enhance cartilage regeneration, a 3D culture system using a natural cartilage scaffold has been developed to create a more chondrogenic secretome. This study investigates the chondrogenic differentiation of mesenchymal stem cells (MSCs) cultured in a decellularized bovine cartilage scaffold and analyzes the resulting secretome's composition. Methods: This study employed a randomized time series design to examine MSCs chondrogenic differentiation. A control group was cultured in standard 2D conditions, while two experimental groups were cultured in either 2D medium supplemented with chondrocyte differentiation medium (positive control) or a 3D decellularized bovine cartilage scaffold.

Low Oxygen Concentration Enhances Chondrogenesis and Secretome Production in Mesenchymal Stem Cells Cultured in a Three-Dimentional Decellularized Bovine Cartilage Scaffolds

Abstract Introduction: Stem cells hold promise for tissue repair due to their secretome, which is influenced by the microenvironment. To improve cartilage regeneration, a three-dimensional (3D) natural cartilage scaffold system has been developed to create a more chondrogenic secretome. As human cartilage is avascular, a hypoxic environment may better mimic in vivo conditions. This study investigates if oxygen levels (normoxic vs. hypoxic) affect MSC chondrogenic potential and secretome composition in this 3D scaffold. Methods: This study used a randomized time series design to investigate how oxygen levels affect the transformation of MSCs into cartilage cells within a 3D cartilage scaffold. A control group (C) was grown in a standard medium. Two experimental groups, P1 and P2, were cultured n a 3D cartilage scaffold under normal (21%) and hypoxic (5%) oxygen concentration. respectively.