Hematological Profile of Mice After Ethyl Acetate Extract of Fungus Comb of Indo-Malayan Termite (Macrotermes gilvus Hagen) Mound Supplementation in Regulating Lipopolysaccharide-induced Inflammatory Response

ABSTRACT Introduction: Termitomyces sp. is an edible fungal species commonly found in tropical forests in Africa and Southeast Asia. It is consumed as an immunomodulatory agent to enhance the immune system. This study explored the hematological profile of ethyl acetate extract of fungus comb(EAEFC) on mice’s inflammatory response induced by intraperitoneally-injected lipopolysaccharide. Methods: An experimental study with a post-test-only control group design using BALB/C mice(n = 24, bodyweight/BW:25-30 grams) was conducted. The animals were randomly allocated into 4 groups, each consisting of 6 mice, and received substance(s) via gavage. Groups I and III received 5% Dimethyl Sulfoxide(DMSO) in water, groups II and IV received 500mg/kg BW EAEFC in 5% DMSO. On day 15, Group I and II were injected intraperitoneally with 5 ml/kg BW saline, whereas Group III and IV with 10 mg/kg BW lipopolysaccharide(LPS) in 5 ml/kg BW saline. After three hours, the mice were sacrificed; their blood was collected, and a hematological profile was observed. The results were displayed as mean ± standard deviation (SD), and the differences between groups were statistically analyzed using one-way ANOVA.