Effect of Ethanolic Extract of Cayratia trifolia on Histologically Kidney Mouse Model

Abstract - This study was conducted to determine the effect of Cayratia trifolia ethanolic extract on histological mouse kidney models. A total of 12 adult male Balb/c mice were randomly divided into two groups, comprising six mice in each group. The first group of 6 mice provided as the control group and received 25 ml/kg BW orally sodium carboxymethylcellulose during 6 days.The second group, 6 mice was administered 50 mg/kg BW of C. trifolia ethanolic extract in sodium carboxymethylcellulose orally during 6 days. The present study, we have performed histological kidney mice evaluation after 6 days of C. trifolia ethanolic extract administration. The histological mice kidney was quantified in term of tubular epithelium, glomerular and focal mononuclear infiltrate. The effect of administration of C. trifolia ethanolic extract on histological kidney of mice appeared normal tubular morphology in cortical and medullary regions of kidney in administered C. trifolia ethanolic extract mice, there was no kidney change histologically showed normal architecture of the glomerulus and tubules, while in control mice group, kidney histologically first marked with mononuclear cell infiltration, tubular necrosis, glomerular congestion and hyaline casts. The study results exhibit that relatively sufficient in histopathological changes in the corticomedullary junction of mice kidneys, including tubular epithelial damage, tubular dilatation and intratubular cast formation.

Keywords: Balb/c mice, C. trifolia ethanolic extract, kidney


1. Introduction

Several natural subtances are possessed the medicinal plants, one of those medicinal plants is C. trifolia. A large number of compounds contained in C. trifolia plants include bioactive compounds such as yellow wax oil, steroids, terpenoids, flavonoids, and tannins [1]. Phenols, flavonoids, and tannins are gaining attention because they have natural qualities in preventing disease, promoting health, and antiaging substances as natural antioxidants [2]. Antioxidants can function to reduce oxidative damage caused by free radicals and reactive oxygen species under conditions of oxidative stress in humans [3]. Several parts of C. trifolia such as stems, leaves, and roots are reported to have hydrocyanic acid and delphinidin. Leaves of C. trifolia have been reported to contain several flavonoids such as cyanidine [1].

Kaempferol, myricetin, quercetin, triterpenes and epifriedelanol are contained the aerial parts of the plant; the seeds and fruits contain cyanogenic compounds; and the leaves also contain stilbenes such as piceid, resveratrol, viniferin and ampelopsin [4]. Patients with diabetes in order to check sugar levels of blood traditionally can be given infusion of seeds along with extract of tubers orally. In several cases such as diuretics, tumors, neuralgia and splenopathy can be medicated to used the whole plant [5]. The presence of alkaloids and flavonoids in the ethanolic extract of C. trifolia can be used for good free radicals scavenging activity. Antioxidants

that contained the ethanolic extract of C. trifolia exhibits a good free radical scavenging activity [6]. Many of the properties of this plant have been reported in previous studies such as antibacterial, antifungal, antiprotozoal, antiviral, hypoglycemic, anticancer, antioxidant, anti-inflammatory and diuretic properties. This study aims to know indirect effect of antioxidants and anti free radical scavenging activity of the C. trifolia ethanolic extract by inducing sodium carboxymethylcellulose that can influence histologically kidney feature in mouse model.